Chronic Hepatitis B (HBV) is the leading cause of morbidity and mortality worldwide with about 248 million people having HBV infection. Trace elements e.g. copper (Cu), zinc (Zn), selenium (Se) and iron (Fe) are constituent components of many metal proteins and metalloenzymes in human sera. Therefore, the ratios of these trace elements in human sera are often stated to be a good marker for diagnosing various diseases including HBV. The aims of this study are: to compare the level of trace elements in sera of patients infected with HBV and healthy participants, and to evaluate the efficiency of analytical techniques (e.g. Inductively Coupled Plasma- Mass spectrometry (ICP-MS), Atomic Absorption Spectroscopy (hydride generation) (AAS) and Graphite Furnace Atomic Absorption Spectroscopy (GFAAS) that are currently used to detect Fe and Se elements in Patients' human sera. The findings of this study show that the concentration range of copper element between (132.80 ±28.64 μg/dl) to (105.66±23.20 μg/dl) was significantly higher in HBV infected patients as compared to those in healthy controls (91.27± 9.20 μg/dl). Iron concentration range between (206.64±61.60 μg/l) to (170.00±36.71 μg/l) was significantly higher in HBV infected patients as compared to those in healthy controls (158.00±15.13 μg/l). However, patients with HBV had significantly lower serum concentrations of zinc with a concentration range between (111.64±20.90 μg/dl) to (99.25± 24.06 μg/dl) as compared to those in healthy controls (113.44±16.38 μg/dl). While selenium concentration range between (64.39±7.39 μg/l) to (51.10±4.96 μg/l) was significantly lower in HBV infected patients as compared to those in healthy controls (67.68±7.60) (μg/l). Moreover, the results of this study suggest that (AAS) technique was the most accurate method to measure the concentration of selenium element, while (UV and ICP-MS) analytical techniques have the same efficiency in measuring the iron concentrati
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