Embryotoxicity of Poorly Soluble Nanoparticles at Various Stages of Zebrafish Development

摘要

The biological effects of the poorly soluble nanoparticles (NPs) of different chemical nature and structural characteristics were evaluated. It was established that the Zebrafish test response to contamination of aqueous medium with nickel NPs (nNi), platinum (nPt), zinc oxide (nZnO) and cerium oxide (nCeO_2) depends on the physicochemical properties of the NPs and embryo development stage. The concentrations of NPs not causing disruptions in embryonic development of Zebrafish were determined. The smallest impact on embryogenesis was exerted by nCeO_2: coagulation of a small number of embryos was observed only at C= 20.0 mg/L. The same effect was observed when exposed to lower concentrations of nPt (C= 5.0 mg/L) and nNi (C= 0.1 mg/L).The greatest number of coagulated embryos was observed when grown in the DS nZnO: 37.5% of embryos died at the DS concentration of C= 0.1 mg/L. Zebrafish cultivation in the DS with low concentrations (C < LC_(10)) of nNi and nZnO caused distortions in the development of embryos: development of scoliosis, malformation of somites, inhibited mobility.