SCALED-UP EXPANSION OF EQUINE CORD BLOOD MESENCHYMAL STEM CELLS (MSCS) FROM 10ML STIRRED SUSPENSION BIOREACTORS TO 100ML COMPUTER CONTROLLED STIRRED SUSPENSION BIOREACTORS USING COMPUTATIONAL FLUID DYNAMIC MODELING

摘要

Musculoskeletal injuries are the leading cause of lameness and loss of performance in horses and conventional treatments are often associated with high rates of re-injury. Mesenchymal Stem Cells (MSCs) have shown promise for the treatment of such injuries in horses. Currently, the majority of studies are focused on the use of either bone-marrow derived or adipose-derived MSCs. However, equine cord-blood derived MSCs (eCB-MSCs) also provide a promising alternative, due to their high proliferation potential, ability to differentiate towards the chondrogenic lineage, and comparable immune-modulatory properties.