Methods of isolation and culture expansion of stem cells / mesenchymal stem from umbilical cord blood, and differentiation method of stem cells / mesenchymal-derived stem cord blood in various mesenchymal tissues.

摘要

A method for the isolation, cultivation and chondrogenesis of mesenchymal stem / progenitor cells from umbilical cord blood, comprising the steps of: Pour the cord blood in Ficoll-Hypaque solution; Centrifuging the umbilical cord blood on the Ficoll-Hypaque solution to obtain mononuclear cells; Reacting cells obtained by monolayer culture of the mononuclear cells with antibodies to specific antigens mesenchymal stem / progenitor cells for a predetermined period of incubation time; Isolating only cells bound to their corresponding antibodies using a cell sorter; Cultivating the isolated cells; and culturing in cell differentiation medium for a predetermined period of incubation time stem cells / progenitor cells for the differentiation into chondrocytes.