In Vivo Suppression of Solid Ehrlich Cancer via Chlorophyllin Derivative Mediated PDT: An Albino Mouse Tumour Model

摘要

In this study, copper chlorophyllin was used as a photosensitizer for photodynamic therapy (PDT) in Ehrlich tumour mouse model. Six groups of animals comprising 5 animals per group were subcutaneously transplanted with 1×10~6 Ehrlich tumour cells. A single dose of 200 μg/gm body weight chlorophylin derivative was administered by intravenous (Ⅳ) or intratumoral (IT) routes. Mice were exposed to monochromatic red laser of 630 nm for 1 h, and tumour regression was followed up for three consecutive months post treatment. Several Biochemical, histological tests were performed in order to evaluate the efficacy and safety of the applied treatment. PDT-treated animals via either the Ⅳ or IT routes showed significant decrease in tumour size 72 h post-treatment. Tumours at the IV-PDT group disappeared totally within a week with no recurrence over three months follow up. In the IT-PDT, the decrease in tumour size at the first week was interrupted by a slight increase; however never reached the original size. Histological examination of tumour tissues of the Ⅳ-PDT group at 24 h post treatment demonstrated restoring the normal muscle tissue architecture, and the biochemical assays indicated normal liver functions. In conclusion Ⅳ-PDT strategy proved better cure rate than the IT-PDT, with no recurrence over three months of follow up.