Survey of Estrogenic Activity of Organic Extracts from Water Body from Jilin City Reach of the Second Songhua River

摘要

Objective: To survey estrogenic activity of Environmental estrogens (EEs) pollution in Songhua River water of the second Songhua River. Methods: human breast cancer MCF-7 cell proliferation experiment were used to detect estrogenic activity of organic extracts of organic extracts from water body. Results: MCF-7 cell proliferation rate increased at first and later decreased with the organic extracts concentration. Bcl-2 mRNA expression of MCF-7 cell increased and Bax mRNA expression decreased with the concentration of organic matter. Cell proliferation was the fastest in organic extracts group equivalent to the raw water 1000 mL. Conclusion: The organic extract could promote cell proliferation through the regulation of Bcl-2 and Bax expression. The harm risks on the human body of the organic matter in water still need further research on environmental toxicology and epidemiology.