VALIDATION OF FUSARIUM HEAD BLIGHT RESISTANCE QTLS IN WHEAT USING DOUBLE HAPLOIDS DERIVED FROM FOUR-WAY CROSSES

摘要

Fusarium head blight (FHB), caused primarily by Fusarium graminearum, is one of the most devastating plant diseases in the world. Specifically in wheat, FHB has become responsible for significant economic and health concerns worldwide due to mycotoxinaccumulation in infected grain, as well as yield and quality losses. To date, sources of resistance conferring complete resistance to FHB have not been identified in wheat. Thus, extensive research efforts worldwide has focused on development and use ofresistant cereal cultivars for the control of FHB. QTLs for FHB resistance have been mapped to almost all wheat chromosomes when different mapping populations were investigated. In our research, we are using double haploid (DH) wheat lines derived fromselected four-way crosses combining several sources of resistance to validate putative QTLs (Xmc758. Gwm33, xbacrl76, Xgml20, Xwmc317, Xwmc332, Xwmc522 and Xwmc296) that could minimize the threat of FHB including the reduction of mycotoxins, to the producers, processors, and consumers of wheat. We use molecular techniques to validate DH lines and their corresponding parents. In this study, we report on our work on the DH derived lines screen for FHB in three northern plains location. Our finding will assist ongoing efforts aimed to develop resistance wheat varieties, minimize the impact of the disease, and provide resources that can possibly assist in the advancement of wheat germplasm research.