Sensitive, Specific and Highly-Multiplexed Detection of Genetic Variation

摘要

There is an increasing need for the sensitive and specific molecular analysis of multiple targets. Mutations in genes such as RAS, RAF, EGFR and PIK3CA are present in a number of cancers including colon, lung and breast cancers. The mutation status of multiple genes may be required for the diagnosis and prognosis of cancer, or for predicting response to targeted therapies. Due to the heterogeneous nature of solid tumours and/or the need to detect rare mutations in liquid biopsies, it is important that detection methods are highly sensitive and specific, and can interrogate multiple targets simultaneously so as to maximise the information obtained from each sample.