Pushing the Limits of PCR, qPCR and RT-PCR Using Hot Start dNTPs

摘要

In a field where the demands for speed, sensitivity and specificity are high, PCR performance can be significantly improved by the use of Hot Start technologies. Hot Start dNTPs are a unique, universal approach that employ nucleoside triphosphates modified with a thermolabile protecting group. This modification blocks ambient temperature primer extension and is gradually released at higher temperatures to allow for more specific DNA polymerase incorporation.