Current emerging platforms for detecting rare biomarkers and tumor cells in the bloodstream have increasingly improved diagnostic sensitivity. Therefore, early signs of metabolic changes can be detected even with a tiny amount of biosample. Precisely manipulating and dispensing small-volume samples is one of the most crucial steps to acquiring reliable results. In particular, when the collected volume is extremely limited, the need for precise dispensing of submicroliter and nanoliter quantities is quite important. To achieve highly reliable diagnosis with a limited volume of blood sample, we developed a positive-displacement high-precision dispensing method by using a nickel electroformed nozzle with an inner diameter accuracy of 5 um or less. When a dispensing variation of 100 nL was evaluated by using a photometric method, the volume error of the dispensing system was 0.7%, i.e. 0.7 nL
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