Assessing the Osteogenic Differentiation of Human Mesenchymal Stem Cells Co-Cultured with Human Vein Endothelial Cells on a Peptide Amphiphile Nanomatrix

摘要

This study has shown that PA nanomatrices have the potential for being used in bone tissue engineering applications. In addition, co-culturing hMSCs and HUVECs on the PA-RGDS nanomatrix was shown to promote the differentiation of the hMSCs and induce the formation of endothelial cell networks. The PA-RGDS nanomatrix further supported the growth and function of hMSCs and HUVECS. The co-cultured cells on the PA-RGDS nanomatrix had the greatest ALP activity, the largest fraction of each surface occupied by mineralization, and the greatest evidence of endothelial network formation.