Intracellular Trafficking of Alginate-graft-Poly (Ethylene Glycol) Microspheres for Promoting Mesenchymal Stem Cell Osteogenic Differentiation

摘要

By utilizing fluorescent labeling of Alg-g-PEG and Alg-g-RGD microspheres, we were able to determine cellular internalization efficiencies. The surface-conjugated ligand peptide RGD increased microsphere internalization, providing a better strategy for intracellular VEGFA delivery for osteoporosis treatment. Additional assays are ongoing to determine the location of entrapped microspheres. Fluorescently-labeled microspheres may also be applied as imaging probes for various applications.