Neuroprotective effects of Smad7-siRNA on oxygen-glucose deprivation (OGD) in PC12 cells

摘要

Ischemic cerebrovascular disease is a global health problem. According to the World Health Organization, ischemic stroke is actually the most common cause of death in the world. ActA/smads signaling pathways were shown to be required for the differentiation-associated physiological apoptosis of stroke. Smad7 is an important regulator of ActA/smads signaling via a negative feedback circuit but its effects have not been well understood. This experiment mainly discussed the apoptosis in ischemic cerebral injury by targeted silence smad7. In this study, we used NGF and oxygen-glucose deprivation (OGD) to stimulate PC 12 cells and convert them into neurons in order to establish an ischemia in vitro model. Combined with the small interfering technology of smad7, we took FCM and DAPI to identify apoptosis rate. These results showed that OGD 16 h apoptosis rate is 19.54%. OGD 16 h combined Smad7-siRNA apoptosis rate is 12.34 %. This results showed that the apoptosis rate was decreasing in ischemic injury when Smad7-siRNA. At the same time, it provide the reference for further study ActA/smads signaling pathways on acute ischemic brain damage.