Optimization of Pre- and Post-Transfection Parameters for Multi-Gram Level CHO Antibody Production Within Two Weeks

摘要

Flow electroporation provides a means of fully scalable, highly efficient transient gene expression that results in antibody titers >1 g/L and production of multiple grams of antibody from non-engineered CHO-S cells. Pre-transfection cell health, growth, and passage number can affect transfection efficiency and cell viability levels and, thus, antibody production. In this poster data using flow electroporation via the MaxCyte STX~R Scalable Transfection System are presented that examine the effects of pre-transfection parameters such as media supplementation, cell density, and cell passage number on CHO-based antibody production. In conjunction, data illustrating the effects of post-transfection parameters such as seed density, media formation, and feed strategy are shown. Taken together these data will demonstrate the ability of MaxCyte STX electroporation to i) produce secreted antibody titers >1 g/L, yielding multiple grams of antibody within two weeks of a single CHO transfection, ii) scale-up without protocol reoptimization while maintaining transfection performance, and iii) enable fine-tuning of pre- and post-transfection parameters to balance speed, cost, and antibody yield. In summary, these data support the use of flow electroporation via the MaxCyte STX as an enabling technology that ultimately reduces early-stage labor and cost expenditures.