Optimization of the Submerged Fermentation Conditions of Ganoderma lucidum with High Triterpenoids Production by Response Surface Analysis

摘要

In this paper, yeast extract concentration, incubation days and inoculation was made as independent variables, the production of intracellular triterpenoids, intracellular polysaccharides and mycelia biomass were made as response values, we set up central composite design of three factors and three levels. Through the analysis of the regression model, we could see that when yeast extract concentration was 1.91%, the inoculation was 14.99% and the incubation days was 4.24 d, the production of intracellular triterpenoids, intracellular polysaccharides and mycelia biomass was 100.654 mg/100 ml, 58.5968 mg/100 ml, 2.39258 g/100 ml, respectively.