Resonance Rayleigh Scattering Effect of Immunonanosilver Aggregations and its Application to Rapid Determination of Trace Hg(II)

摘要

Using PEG-10000 and sodium citrate as stabilizer, and NaBH_4 as reducer, a stable nanosilvers (AgNPs) sol was prepared. In pH 6.6 phosphate buffer solution containing NaCl, the AgNPs were aggregated to large particles, which lead to resonance Rayleigh scattering (RRS) peak at 350 nm enhancement. Upon addition of cysteine, the peak decreased. The decreased value △I is linear to cysteine concentration in the range of 5-60×10~(-8) mol/L. Thus, a new RRS method was proposed for detection of cysteine.