Discrimination of Target Proteins Using Array Sensor with Liposome Encapsulating Fluorescent Molecules

摘要

This paper reports a new type of detection approach by arrayed biosensor for discrimination of target biomolecules such as protein and/or evaluation of their concentrations, utilizing liposome encapsulating fluorescent molecules and the corresponding time course analysis of the fluorescence. This method is realized by obtaining multi-dimensional data plots (curve) of fluorescent output such as its intensity from plural and different liposomes independent from each other. In this work we discriminated the species and concentration of target proteins by the method: Two different proteins (carbonic anhydrase from bovine: CAB and Lysozyme), including their different concentrations were clearly discriminated. In addition, the extent of discrimination was improved by denaturation of the protein, as the denatured protein strongly interacts with liposome membranes because of the change in conformation states of protein.