MUTANTS SHEWANELLA ONEIDENSIS MR-1 EFFECTIVE IN GENERATING BIOELECTRICITY IN MICROBIAL FUEL CELLS

摘要

The capability of Shewanella oneidensis MR-1 mutants FRS1 and FRB1, characterized by a high reducing activity, to generate electricity was measured in microbial fuel cells (MFC) of different types. The reducing activity of these mutants was previously identified with respect to the rate of discoloration (reduction) of methylene blueё, a dye that served as an electron transfer mediator. The present study demonstrated that the rate of MB discoloration was in direct correlation with the level of voltage in MFC of the above strains. In case when the mutants were used, the parameter in MFC proved to be 30-40% greater than in the case of the parent strain. In the course of this investigation MFC characteristics were identified, optimal electrode materials and ion-selective membranes selected, and MFC laboratory mockups manufactured.