Effect of Composition Changes on Sensitivity of Glucose Sensitive Hydrogel Studied by Design of Experiments (DOE)

摘要

Currently, the most promising sensing technology for devising a body implantable glucose sensor involves analyte-stimuli responsive hydrogels because of biocompatibility, non-toxicity, and inflammability. Hydrogel containing phenylbronic acid (3-APB) evinced good sensitivity and selectivity to glucose molecules.1 3-APB moieties in hydrogel perform a role as a reversible cross-link, forming dual binding structures with cis-diol of glucose molecules, but only producing single binding structures with fructose molecules. However, there are still several problems should be solved such as sensitivity, and response time. Scaling down to micro size, and confirming of the relevance of chemicals are necessary to develop function of glucose sensitive hydrogel (GSH). In this research, a statistical technique such as design of experiment (DOE) was used for getting reliable results with minimum samples. DOE has been used for industrial field to enhance reliability of products and increase yield within limited cost and time. Thus, for this study, DOE was run by 3 factors with continuous level because continuous level study is useful to confirm response tendency depending on factors. And, two types of responses such as magnitude and 1st order rate were used for analysis whereas most of hydrogel DOE studies are using one type of response such as water absorption rate.2,3 Experiment results showed that sensitivity of GSH was largely depending on the ratio of APB/DMAPAA, and pre-gel solution wt%. And, the first order rate was mostly affected from the ratio of AAm/BIS. All hydrogel samples were synthesized by UV polymerization method for setting in-situ synthesis up to scale down.