Polyacryionitrile-glycopolymer Nanofibrous Membranes for the BSA Adsorption

摘要

Membranes are fast growing for bioseparation techniques,however,they are easy to be polluted by proteins and many other components existed in the mixture.Carbohydrates are ubiquitous on the extracellular surface of living cells and mediate a myriad of biological recognition and signal processes and also can inhibit the adsorption of non-specific proteins.Glycopolymer nanofibrous membranes offer a new method to mimic cellular events.We describe a simple and effective method of constructing glycosylated membrane surfaces using Poly (6 O-Vinyladipoyl glucose) (Poly-OVSEG) by a water phase precipitation homopolymerization process using the polyacrylonitrile (PAN) as the supports.The structure was characterized by Fourier transform infrared spectroscopy (FT-IR) and Nuclear magnetic resonance (NMR).Composite membranes of PAN/poly-OVSEG nanofibers containing varying amounts (40％ ～60％) of poly-OVSEG were subsequently prepared using electrospinning.The nanofibers were studied by scanning electron microscopy (SEM).Bovine serum albumin (BSA) was used as probes to study the nonspecific interaction on this surface to proteins.The result indicated that the novel glycopolymer nanofibrous membranes in this work had well hydrophilicity and biocompatibility than PAN membranes which could prevent undesirable nonspecific adhesion of foreign proteins.