Induced Synthesis of Hydroxyapatite by Chitosan for Enamel Remineralization

摘要

The formation of organized nanocrystals that resemble tooth-like hydroxyapatite is crucial for successful enamel remineralization. Based on the principles of biomineralization, spindle - shaped hydroxyapatites (HA) were synthesized through biomimetic method with chitosan as template under a controllable way in vitro. We observed that hydroxyapatite nanorods can be controlled followed by in situ crosslinking process and triggered by conditions of pH and ionic strength. The dentinal tubule were blocked by neonatal hydroxyapatite layer and this composite a continuous structure of columns crystal with size of 10-40nm. At the same time, XRD showed that the precipitation was calcium fluoride phosphate and Ca:P was 1.6. Furthermore, there were column crystal with parallel direction inside, as same as the crystal array in the top of enamel rod. The results suggest that chitosan monolayer may be useful in the modulation of mineral behavior during in situ dental tissue engineering.