One-step antibody immobilization-based high sensitivity immunoassay procedure for potential in vitro diagnostics

摘要

A potential one-step antibody (Ab) immobilization strategy was developed for the leach-proof binding of capture Ab to the polystyrene microtiter plate (MTP) [1-3]. It enables the development of high sensitivity, cost-effective and analytically superior immunoassay (IA) for potential in vitro diagnostic (IVD) kits. The NIF-based IA detected HFA in the range of 4.9 pg mL~(-1)-20 ng mL~(-1) with limit of detection (LOD) of 7 pg mL~(-1) (Fig. 1B (a)). The analytical sensitivity was 10 pg mL~(-1), which was 51-fold more sensitive than the convention immobilization format (CIF) based commercial ELI-SA.