Wide-field fluorescence sectioning microscopy using dynamic speckle illumination

摘要

In this paper, we present a novel wide-field fluorescence sectioning microscopy using dynamic speckle illumination that provides depth discrimination. Some simple modifications are made to a conventional wide field fluorescence microscope, which allows for the illumination of the sample using dynamic speckle patterns and the acquisition of a sequence of independent wide-field fluorescence images. The full-field fluorescence image of the sample at a particular depth is obtained using specific digital image processing algorithms. Compared with conventional confocal scanning fluorescence microscopy, this novel imaging modality is capable of providing a reasonably high sectioning performance with a compact and cost-effective system. The experimental results show that the wide-field fluorescence sectioning microscopy can provide high contrast imaging even in scattering biological media, therefore has wide potential applications in biomedical research and clinics.