Visible component of sunlight has a physiologically significant effect on human skin and long-term exposure toconcentrated blue light energy (sunlight, laptop, cell phones) could produce oxidative stress leading to the premature skinaging. In this work, in vivo confocal Raman spectroscopy was used to characterize biochemical changes in human skinafter been irradiated with different doses of blue light. After ethical committee approval, volunteers’ phototype Ⅰ and Ⅱ(Fitzpatrick classification) have been selected. The River Diagnosis confocal Raman spectrometer was used, before (T0)and after 15, 30 and 60 minutes of blue light irradiation (LED 450 nm) with doses of 100 J/cm~2. It was possible to evaluatethe biochemical skin damage caused in the stratum corneum and in the viable epidermis.
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