A New Method for Isolation and Culture of Mouse Uterine Smooth Muscle Cells

摘要

A new method for isolation and culture of mouse uterine smooth muscle cells was established. First, uterine smooth muscle was separated by mechanical separation. And then type I collagenase and trypsin were used to isolate uterine smooth muscle cells. More than 90% of the dissociated cells exhibited initial viability. The surviving cells were observed and verified by immunofluorescence staining. After 3 days of incubation, primary cultures of cells attached the wall of the incubation dishes, and reached confluence with 2 weeks. The cells grew with spindle-shape and showed typical "hill-valley" pattern. Immunofluorescence staining for smooth muscle a-actin shewed positive reaction in more than 98% attached cells. The cells cultured in this experiment survive well, with highly purity, and can serve the further studies of physiology and pathology.