The Effect of Quercetin for Salt Induces Renal Fibrosis by Suppressing Collagen Production

摘要

The bad effect of salt have been suspected for a least one hundred years. High salt intake increased production of TGF-β whereas TGF-β activates a receptor serine/threonine kinase (RS/TK). Through phosphorylation Smad2/3 and Co-Smad cascade, they recruited transcription factors and co-activator for initiates gene transcription for cell proliferation, cell differentiation, and extramatriks cellular production include collagen. This mechanism initiates renal fibrosis. This study was quasi-experimental research with post-test only group design. A subject was 24 male Wistar rat induced with two %/kgBB doses of NaCl 8%. The subjects were divided into six groups. NaCl 8% and quercetin were delivered by gavage daily for eight weeks. After treatment the kidneys were taken for quantification the collagen volume fraction were stained with picrosirius red then quantification by Image J software. The level of Glomerular volume collagen fraction group OO (3.78±0.12%), NO (19.05±0.99%), NK5 (19.42±3.19%) NK10 (17.14±0.70%) and NK20 (3.22±0.81). Tubular volume collagen fraction group OO (2.26±0.08%), NO (9.79±0.53%), NK5 (9.72±0.98%), NK10 (5.25+0.49%), NK20 (1.92±0.20%). Our data indicate that quercetin may be suppressing the collage volume fraction with a significant difference in the dose of 20 mg/kg BB quercetin.