Colorimetric detection of acetylcholinesterase using paper hybrid centrifugal fluidic on disc platform

摘要

We developed the acetylcholinesterase detection system using paper hybrid centrifugal fluidic disc platform. A new method of metering using paper was applied to disk platforms. After the solution is applied to the paper, a certain amount of solution flows to the detection zone according to the rotation speed and rotation time of the disc. Detection zones contain various concentrations of pesticide and material for colorimetric detection. Therefore, the buffer-injected through the inlet is distributed in the same amount in 6 detection zones to activate the pre-contained enzyme. The disk platform was configured to obtain a calibrated curve for the concentration of acetylcholinesterase. Pesticides inhibit acetylcholinesterase and cause a cholinergic overdose. Blood pesticide detection is a method of measuring acetylcholinesterase activity. Therefore, a calibration curve that is detected in the same environment is needed to diagnose the activity of the enzyme due to pesticide poisoning of blood. The assay consists of acetylthiocholine and ELLMAN reagent. Acetylcholinesterase decomposes acetylthiocholine into thiocholine and ELLMAN reagent develops yellow color by the product. The color change of the detection zone was obtained through scanner after 2 minutes of buffer injection. In this study, the concentration of the enzyme was detected by metering through paper without any elaborate plastic processing or chemical treatment of the channel. The platform can provide a suitable standard for detecting suspected blood and pesticide substances on the same disk platform.