Microscopic Urinalysis by Digital Holographic Microscopy

摘要

Urine analysis (urinalysis) is a critical component to diagnose urinary tract disease. Microscopic evaluation of the urineprovides an insight into potential underlying urinary tract disease, which is used for identification and characterization ofboth common and much less common formed elements in the urine sample. In this paper, the microscopic urinalysis ispresented by using single beam digital holographic microscopy (DHM). This is a common path set up wherein bothbeams (reference and sample) travel through a similar path providing higher temporal stability. In this paper, phasecontrastthree - dimensional imaging of red blood cells (erythrocytes), white blood cells (leukocytes), squamous and nonsquamousepithelial cells, casts and various crystals present in the urine samples, have been demonstrated. The proposedimaging modality for the diagnosis of urinary tract disease is simple, non-contact, non-invasive, and provides highertemporal stability due to its common-path geometry.