A new frequency-shifting confocal microscopy (FSCM) illuminated by an azimuthally polarized Bessel-Gaussian (BG)beam is investigated. The solid excitation spot is produced by the BG beam modulated by a spiral phase plate, and thedonut excitation spot is directly obtained by the same BG beam. Through vector diffraction theory and two-view RLreconstruction algorithm, the optical transfer functions of two confocal imaging modes and the simulation imaging ofFSCM are presented. The results show that, two illumination modes produced by the azimuthally polarized BG beam canenhance the spatial resolution of FSCM, the spatial resolution of reconstructed image is mainly depended on theillumination mode with higher frequency transfer efficiency, the small pinhole is helpful to improve the contrast andspatial resolution of image. When the iterations number is about 100, the reconstructed image has good quality. ThisFSCM is helpful to quickly realize super resolution and high contrast in cell imaging.
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