Intracellular Trafficking Characteristics for Magnetic Layered Double Hydroxide/DNA Hybrids to Human Gastric Cancer Cells

摘要

We report here the intracellular trafficking characteristics of a gene delivery system composed by magnetic layered double hydroxide/DNA (MLDH/DNA) hybrids to human gastric cancer cells (SGC-7901). Perls Staining of MLDH/DNA was done to examine the intracellular iron uptake and accumulation; confocal microscopy and transmission electron microscopy were evaluated the cellular internalization feature of the MLDH/DNA system. We have indicated that the MLDH/DNA hybrids are able to enter into the nucleus within 2 h of incubation, displaying a prospect for application in targeted gene delivery.