Spermatozoa Motility of Giant Grouper (Epinephelus lanceolatus) 48 Hours: The Role of Soybean Milk as a Cryoprotectant

摘要

The purpose of the study was to investigate the spermatozoa motility of giant grouper (Epinephelus lanceolatus) 48 hours after frozen. The sperm was gathered by hand stripping method and then frozen at -20 °C after diluted by soybean milk. The various amounts of soybean milk used in this experiment were 5 %, 10 %, 15 %, 20 % and 25 %, respectively. Data were analyzed statistically either by ANOVA or Tukey test. The result showed that soybean milk had a significant effect (P < 0.05) in protecting spermatozoa after frozen. The highest percentage of spermatozoa motility (81.17 ± 1.92 %) was shown by 15 % of soybean milk. Therefore, 15 % of soybean milk was the optimum concentration to protect giant grouper spermatozoa.