The Effect of Different Concentrations of Ethylene Glycol and Skim Milk in Vitrification of Ovarian Rat (Rattus norvegicus L.) Strain Sprague-Dawley

摘要

Vitrification as a part of the assisted reproductive technology (ART) has become the main concern of reproductive organ preservation. Vitrification is a development of reproduction biotechnology to overcome reproductive problems. Ethylene glycol (EG) as intracellular cryoprotectant has been commonly used in rat ovary vitrification, while skimmed milk (SM) has been previously used as extracellular cryoprotectant in fish sperm cryopreservation. The research is aimed to find the best concentration among 3.75 %, 7.5 % and 15 % of EG and SM in preventing ovarian tissue 48 h after vitrification. The selected 21 ovaries were used in this study. The samples (n = 21) were divided into 7 groups. Control group (NC 1, NC 2, NC 3, n = 3) includes sample without vitrification. Control treatment group (TC 1, TC 2, TC 3, n = 9) includes sample that vitrificated with EG in concentrations of 3.75 %, 7.5 % and 15 %, respectively. Treatment group (T 1, T 2, and T 3; n = 9) includes samples vitrificated with EG and SM mixture in concentrations of 3.75 %, 7.5% and 15%, respectively. The results showed that the weight of the ovarian tissue pre-vitrification and post-vitrification in TC 1, TC 2, TC 3, T 1, T 2 and T 3 were not significantly different. In conclusion, this research showed that vitrification of rat ovary using ethylene glycol and skim milk mixture may have no effect on weight of ovarian tissue.