Single-cell electroporation is a technique that consists of applying a strong pulsed electric field(PEF)to single cells in order to create a transient permeabilization in the cellas plasma membrane.While this technique could be used more easily for the transport of molecules into cells for a wide range of applications such as CRISPR Cas9 genome editing,it is largely empirical which translates to being difficult to control.It also shows low transfection yields in batch treatment and low cell viability,thereby limiting biomedical applications.Here,we propose a PDMS microfluidic device in which flow-through electroporation can be reliably performed for large volumes of cells.
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