An Effect of Magnetic Beads to Boesenbergia rotunda Antioxidant Activity Using Photoprotective Micofluidie CD

摘要

Microfluidics compact disc (CD) system has offer many advantages to biological and chemical analysis. It simplifies the procedures and miniaturize the sample and reagent volume. Beads has been used in the microfluidics CD technology in order to enhance the mixing of the reagents or sample. However, the effect of the beads need to be monitor to ensure the effectiveness, biocompatibility and corrosion protection of the beads. This paper has presented the advantage of photoprotective microfluidic CD for Boesenbergia rotunda antioxidant activity and the effect of magnetic bead in enhancing the reaction time of the mixing. The results have shown that, with the magnetic beads usage, the uniformity time of the liquid mixing in the reaction chamber are faster than the non-beads reaction chamber. However, in terms of the stability, the beads have been found to give an interference to the Boesenbergia rotunda 2,2-diphenyl-l-picrylhydrazyl (DPPH) antioxidant activity. To solve this issues, we would recommend an inert coating of the beads to prevent a chemical reaction between the beads and the free radical's reagents. The proposed method has minimized human handling in the DPPH plant antioxidant activity by using microfluidics CD with imitation of the real test environment in the conventional method (photoprotective). Consequently, the laborious repetitive routine in the laboratories, which is one of the factor in pre-analytical error has been reduced. This system would be a great advantage to the future laboratories techniques, as it can be applied as a point of care testing and be used in the small laboratories.