DNA QUALITY AND QUANTITY TEST OF SPERM IN FRESHWATER

摘要

Sperm is one of the body fluids that contain DNA and can be used as evidence or guidance to uncover various criminal cases. Evidence such as sperm can be found in various environments depending on the crime scene, such as being on a submerged cloth. The quality and quantity of DNA obtained from sperm is strongly influenced by the environment where the sperm are found. In this study, we tested the quality and quantity of DNA from sperm spots soaked in freshwater. The quality and quantity of DNA was measured using a spectrophotometer with a wavelength of 260nm and 280nm. The results showed an increase in the quality and quantity of DNA from sperm spots soaked on Day 1, 3 and 7. DNA ribbon also appeared on all samples when the DNA was amplified with STR primers at D21S11 and D13S317 locus.