Determination of Bergenin in Yuchuangling Capsules by High Performance Capillary Electrophoresis

摘要

This paper presents the determination of bergenin content of Yuchuangling capsules by high performance capillary electrophoresis (HPCE) method. Elastic quartz capillary electrophoresis separation control at the temperature of 20°C without coating capillary (75μm×44/52 cm), the sample height was 7.5cm, we choose the borax solution as buffer solution, and its concentration was 20 mmol/L at a constant voltage of 20kV and injecting time of 10s. Linearity was kept in the concent ration range of 0.085-1.35mg/mL of bergenin with correlation coefficient of 0.998. Measured bergenin content in Yuchuangling capsules was 15.93 mg/g (RSD = 4.78%) (n = 5). The recovery was in the range of 90.7% -115.1% (n=5). This method is specific, simple and rapid and accurate, which is suitable for the detection of the content of bergenin in Yuchuangling capsules.