Carbon Paste Electrode Modified Molecularly Imprinted Polymer as a Sensor for Creatinine Analysis by Stripping Voltammetry

摘要

Modification of carbon paste electrode with molecularly imprinted polymer (CP-MIP) as a voltammetric sensor for creatinine has been developed. MIP was synthesized by reacting melamine, chloranil and creatinine with a mole ratio of 1:1:0.1. Creatinine was extracted from polymer chain by using hot water to form a specific imprinted for creatinine molecule. Carbon paste-MIP electrode was prepared by mixing activated carbon, solid paraffin, and MIP in a 45:40:15(w/w %) ratio. The optimum conditions of creatinine analysis by differential pulse stripping voltammetry (DPSV) using the developed electrode were the accumulation potential -1000 mV during 90 s at pH 5. The precision of the method for 0.1-0.5 μg/L creatinine was 88.7-96.3%, while the detection limit of this method was 0.0315 μg/L. The accuracy compared by spectrophotometric method was 95.3-103.6%