Resistance to Assistance Characterization to Petroleum Degradation of Moderately Halophilic Bacteria Halomonas sp. B01

摘要

Petroleum is a major pollutant of the marine environment. In order to improve the petroleum hydrocarbons degradation rate of bacterial consortium, we screened strains from a sample of Dalian salt lake sediment and obtained a strain which not only can grow in the 6% NaCl solution LB culture medium, but also synthesize and secrete ectoine. Through the strain morphology observation and 16S rDNA sequence analysis, it was identified as Halomonas and named as Halomonas sp. B01. In 0.5-2 M certain range of salt concentration, the higher NaCl concentration was in the culture medium, the more ectoine Halomonas sp. B01 producted, ectoine yield reached to 2293.7 mg/L induced by 2 M NaCl at 72 h. Ectoine has a significant role in promoting the growth of oil degrading bacteria. Ectoine-excreting strain Halomonas sp. B01 has the resistance to assist the function of bacterial consortium's degradation of petroleum. Using gas chromatography to determine the petroleum alkane degradation rate of bacterial consortium, Halomonas sp. B01 increased the average degradation rate of diesel oil and Daqing crude petroleum alkane component by 12.0% and 15.5% respectively.