An impedance biosensor for simultaneous detection of low concentration of Salmonella serogroups in poultry samples

摘要

An impedance-based MEMS biosensor was designed, fabricated and tested for the rapid and simultaneous detection of three Salmonella serogroups or other pathogens at low concentrations in ready to eat (RTE) turkey. The important features of the device are the existence of the focusing region and the multi-channels to enable the detection of multiple pathogens or serogroups. The performance of the devices was excellent as evidenced by the focusing capability that increased the strength of the measured signal by a factor between 4 and 18, high sensitivity of 7 cell/ml and detection time of 30 minutes which is significantly quicker than traditional bacterial culture, polymerase chain reaction (PCR) and enzyme-linked immunosorbent assay (ELISA) based detection [1,2].