The Lipase-catalyzed Synthesis of Ethyl Ferulate

摘要

Selective biocatalytic synthesis is an important part of the third generation of biotechnology, is the forefront of international scientific and technological development. Utilizing enzyme catalyzed reaction, many complex substances can be synthesized. Biocatalysis have strict regional selectivity and enantioselectivity. Ferulic acid is naturally safe radical quencher and anti-oxidation agent. In the cosmetics industry, ferulic acid is considered to be the best natural sunscreen, also used as skin whitening agents. Ferulic acid and its derivatives are widely used in the pharmaceutical industry, the application in the food industry are also increasing. This paper studied the immobilized lipase-catalyzed synthesis of ethyl ferulate in organic medium. Lipase from Mucor sp.M-2 was immobilized with diatomite powder as the carrier by absorption and was used as biocatalyst. The reaction medium were compared for biocatalytic synthesis of ethyl ferulate. The optimum solvent is tert-butyl alcohol. The opitimization of reaction conditions was obtained. In the 4ml of tert butyl alcohol, when the molar ratio of acid to alcohol is 1.5:1, the temperature is 45 degrees centigrade, a yield of 9.9% for ethyl ferulate can be obtained after 120h.