Study on the of Fungi Antineoplastic Constituents in Root Plant of Acanthus ilicifolius L

摘要

Acanthus ilicifolius L is a kind of pantropical mangrove plant. It is cold-natured and bland. The whole plant is officinal. According to recent studies, Acanthus ilicifolius L has a variety of pharmaceutical function, such as hepatoprotective effect, antioxidant, anti-tumor effect and insecticidal effect. To study the active ingredients of plant fungi of Acanthus ilicifolius L, we focus on the study of anti-tumor active ingredients. We studied the fermentation of hyphostroma of fungal strain LSJ120 in this laboratory which was isolated from the roots of Acanthus ilicifolius L. The ethanol extract, the extratable petroleum ether, the chloroform extract, the ethyl acetate extract, the n-butanol extract, the aqueous extract and 8 hyphostroma fermentation active constituents which was isolated from the ethyl acetate extract can be obtained by silica gel column chromatography. In Y1-Y8 component, 10 compounds were obtained from the separation and purification; Using MTT method, respectively, we do anti-tumor experiment for the extracts and compounds of each group. From the results, The inhibitive rate of the cells was the highest in the ethyl acetate extract. The highest value reached 80.73%; from 8 kinds of components which were isolated from the ethyl acetate extract, the inhibition ratio of the Y6, Y7 components is significantly higher than the other groups; In addition, the compounds purified from the 8 kinds of isolates are also obtained correspondingly. The antineoplastic activity of compound extracted from Y6, Y7 components was stronger. Among them, the inhibition ratio of compound 6, compound 9 was significantly higher than other compounds, which is the main antineoplastic constituents of this fungi. By structure identification, the compound 6 was a cyclopentanone material, and the compound 9 is a cyclic dipeptide derivatives. The finding provides the scientific basis for the development and utilization of the medicinal value of Acanthus ilicifolius L.