Analysis on Separation and Purification of Corosolic Acid and Antitumor Activity

摘要

Objective: To make a study of the separation and purification of purified corosolic acid from loquat leaf extract using the traditional Chinese medicine technology and the separation process in different samples of human gastric carcinoma BGC823 cell growth inhibition. Methods: 95% ethanol extracts were purified by macroporous adsorption resin, silica gel column chromatography and preparative chromatography. The MTT method was used to test the effects of different samples on the growth of BGC823 in gastric cancer cells. Results: corosolic acid can inhibit the growth of gastric cancer cell line BGC823, isolated from different Coro cable acid samples with different degree of inhibition and other components (such as hawthorn acid or mature acid) and the coronation cable acid can produce synergistic effect. Conclusion: the method can provide experimental basis for the establishment of purification of corosolic acid and the development of new anticancer drugs.