Durable 3D test standards from mouse retina for performance testing of high-resolution optical coherence tomography systems

摘要

For performance testing of high-resolution label-free optical coherence tomography (OCT) systems, durable test standardswith optical properties similar to native biological tissues are highly desirable. Therefore, we explored the establishmentof ex-vivo 3D samples from dissected mouse retina. To achieve high stability, dissected tissue parts were embeddedin EPON resin. Results from comparative investigations with OCT on murine retina in agarose demonstrate thatby utilization of appropriate EPON embedding protocols durable samples with adequate refractive index properties canbe created in which even tiny structures near the OCT resolution limits can be detected.