Metabolomic differences in a susceptible and resistant Beta vulgaris germplasm during early responses to Rhizoctonia solani AG 2-2 IIIB.

摘要

Sugar beet can be significantly impacted by Rhizoctonia crown and root rot caused by Rhizoctonia solani AG 2-2 IIIB. The molecular processes that mediate sugar beet resistance to R. solani are largely unknown and identifying the metabolites associatedwith R. solani infection may provide novel targets to utilize in breeding programs for enhanced resistance. The metabolic changes that occurred during susceptible (FC901) and resistant (FC709-2) R. solani interactions were compared with mock inoculatedtreatments and characterized using a non-targeted metabolomics workflow spanning primary and secondary metabolism products. Plants of both germplasm were grown in a greenhouse until 10 weeks after sowing. Hulless barley was infested with R. solani AG 2-2IIIB isolate "R-9", air dried, ground, and then used to inoculate 12 pots, each containing 3 plants, for each germplasm. 12 pots of each germplasm, also containing three plants each, were inoculated with ground un-infected barley as a "mock" infected control. At 0, 3,5, and 7days after infection (dai) or mock inoculated root and leaf tissue were taken from 3 pots for each of the two sugar beet lines, flash frozen with liquid nitrogen and then ground finely with a mortar and pestle. Metabolites were extracted with methanol: water (80:20) and detected using non-targeted reversed-phase UPLC-MS and GC-MS workflows.