In Vitro Susceptibility of Silver Nanoparticles on Thai Isolated Fungus Pyricularia oryzae from Oryza sativa L. Leaves

摘要

Silver nanoparticles (AgNPS) have been known for their effectiveness as antimicrobial agents due to their extremely large relative surface areas increases their contact with bacteria and fungi thus improving its bacteriocidal and fungicidal effectiveness. In this experiment, AgNPs were tested for antifungal activity on a rice blast fungus, Pyricuralia oryzae which is anamorph of Magnaporthe oryzae. In Vitro inhibitory property of AgNPs were observed on the fungi isolated from rice leaves and the survival isolates after AgNP treatment. The results showed AgNPs retarded and reduced the fungal growth at low concentration of 25 μg ml~(-1). The retarded fungi after previously treated with AgNPs gave very slower hyphal growth. The results demonstrated that AgNP can strongly inhibit the fungal growth and colony formation. AgNPs can thus be improved to be promising antifungal agents against Pyricularia oryzae with its suitable formula of AgNP in the fungicidal materials.