Research on Microbial Degradation of Bamboo Lignin for Extraction of Natural Bamboo Fiber

摘要

This study optimizes on the technological conditions for extraction of natural bamboo fiber by means of microbial degradation of bamboo lignin. Results show that by the orthogonal experiment, we have studied out optimal conditions for combining three strains with fermentation to produce enzyme: 10.0 g/L glucose, 0.2 g/L ammonium tartrate, 4.5 buffer solution pH value and 20.0/250 ml loading liquid quantity. In these above four factors, the primary and secondary factors affecting enzyme production are: carbon source > pH value > loading liquid quantity > nitrogen source. Meanwhile, the experiment has studied the influence of single factor on lignin degradation: at the temperature of 37°C, about 4 × 10~7 spore concentration, adding 1.5 mg/L veratryl alcohol and introducing air at the efflux rate of 1 L/min are bamboo crude fiber optimal conditions for microbial selective degradation of lignin.