Analysis of Electrostatic Interaction between Ganglioside GM3 and Transmembrane Peptide

摘要

In recent years, some examples have been reported in which gangliosides control the activity of membrane proteins, particularly RTKs. Insulin receptor is one of them and is known to interact with ganglioside GM3, resulting in the suppression of signal transduction. This interaction has attracted interest because it gives rise to type 2 diabetes by causing insulin resistance. However, there was no way to demonstrate the actual mechanism involved. Therefore, we constructed a model system that can analyze the interaction between insulin receptor and GM3 using liposome. Investigation of lipid ratio of liposome and 3D imaging made enables us to observe slight differences of peptide localization. This model would be useful for detailed analysis of electrostatic interactions.