Gold Nanoparticles and X-Rays Cause Estradiol Hormone Reduction Across Oxidative Stress of Blood Samples in Vitro

摘要

Gold nanoparticles (AuNPs) have been widely used and applied in science, biotechnology and medicine fields because they have many unique properties including their ease synthesis, optical characteristics and chemical stability. The purpose of the present study was focused to investigate the advantages of AuNPs in reduction of estradiol hormone and might employed this phenomenon to treat breast cancer across reducing this hormone and amplification of their effects across irradiation with x-rays. A total number of the subjects included in this study was 15 women and all ages were ranged between 18 to 22 years old. The serum derived from blood samples of women were sub-divided into two groups ; the first group was served as a control group and the second group was firstly mixed with AuNPs for 24 hours and then irradiated with x - rays (5 Gy) .The data obtained from the present study showed a significant decrease (p <0.05) in the concentrations of estradiol hormone of blood samples when mixed with AuNPs compared to non-treated samples ( control group) and these results recorded a progressive lowering ( p <0.05) when the samples -AuNPs mixture irradiated with x- rays ( 5 Gy) in matching with those non-treated samples. Moreover, the same results were found in the activities of catalase enzyme and its results confirmed a remarkable reduction (p<0.05) in both treatments (sample- AuNPs and irradiation of this mixture with x-rays. In contrast, levels of malodialdehyde (MDA) were significantly increased (p<0.05) when samples mixed with AuNPs and then they exposed to x-rays as compared to control group. Concerning the correlations among studied parameters were insignificantly different (p>0.05). In conclusion, the AuNPs have ability to reduce estradiol hormone because of generation of free radicals and drop of antioxidant system and these effects can be amplified and potentiated through exposure to moderate dose of x-rays.