Quantitative Assessment of Nanoparticle Induced Oxidative DNA Damage Using Isotope Dilution Mass Spectrometry

摘要

Hyphenated mass spectrometry techniqus have been employed as one of the primary analytical tools for investigating the effects of ionizing radiation, chemical/biological carcinogens, and oxygen derived free radicals on the induction and subsequnt repair of oxidatively-induced DNA damage (DNA lesions) in living systems. The National Institute of Standards and Technology has established a comprehensive research program focused on identifying mechanisms of DNA damage caused by commercially relevant engineered nanoparticles (NPs) using high resolution mass spectrometry for the quantification of oxidatively-induced DNA damage. We present results from a recently published study on the genotoxicity of National Institute of Standards and Technology (NIST) standard reference material (SRM) gold nanoparticles using isotope dilution liquid chromatography /mass spectrometry analysis.