Whole Genome Sequencing and Biological Characterization of Duck/JS/10,a New Lentogenic ClassⅠNewcastle Disease Virus

摘要

A lentogenic Newcastle disease virus (NDV),Duck/JS/10 (JSIO).was isolated from a non-vaccinated duck in China.The complete genome of the virus contained 15,198 nucleotides.Based on length of genome and sequence of partial F gene,the virus was classified as a class Ⅰgenotype 4 NDV.The antigenicity of the virus was compared with that of NDV strain La Sota via hemagglutination inhibition (HI),virus neutralization (VN)assay and animal experiment.Our results show that JSlO generates higher HI and VN titers than La Sota against hoth class Ⅰ and Ⅱ virulent NDV strains.Experiments on animals demonstrate that virus shedding from chickens vaccinated with JSIO is significantly reduced when compared to those vaccinated with La Sota.Overall,the study strongly suggests that JSIO may qualify as a new vaccine candidate against Newcastle disease.